@ Alessandro
You adjust the flow at the distribution box of the underfloor heating once for each room so that this room reaches its normal setpoint temperature.
At that time, look up thermal balancing to understand the process exactly.
Assuming your setpoint temperature is 21 degrees as in your example.
The room now also has 21 degrees.
Then, simply put, the following happens:
The heating water arrives at the appropriate supply temperature, since the room already has 21 degrees the heating water can no longer transfer heat to the room and flows out again at the same temperature.
The water returns to the heat pump having lost almost no temperature.
Now the water can either go to another room and be used there, or the heat pump detects that no consumption takes place and reduces the output.
The goal with a heat pump is always to run it as long as possible at the lowest possible output.
If you now regulate this via thermostats, the following happens simply:
Heating water runs, thermostat regulates down,
The flow rate is significantly reduced since at least one room is missing.
Here it can already happen that the heat pump goes into fault mode because the minimum flow rate is not reached.
Heat pump switches off because no heat is being drawn.
Thermostat notices the room temperature drops and releases supply, heat pump starts up at full capacity, runs 2-3 minutes and is then throttled again by the thermostat.
And now you have a heat pump that has to make dozens of starts per day, and wears out faster.
The best control is always the internal one of the heat pump or the condensing boiler.